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Theories of staining (2)

  • Occur between reagent and tissue substrates
  • Involves various intermolecular attractions
    • Dipole-dipole
    • Dipole induced dipole
    • Dispersion forces
  • These forces are polar attractions
  • Effective over a short distance
  • Non symmetrical molecules posses stronger dipoles than symmetrical molecules


  • Factors affecting are:
  • Variation in section thickness
  • Temperature
  • Stirring of the reagent solution
  • Presence of cavities in the tissues
  • Dyes are easily lost by permeable structures
  • Impermeable structures retain stain the longest

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